Product Detail

• Sizing and approximate quantification of large DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA

Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer.

This marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow fragment or Klenow Fragment, exo- with the fill-in reaction.

For Research Use Only. Not for use in diagnostic procedures.

Note The cohesive ends (the 12nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5minutes and then cooling on ice for 3minutes.