Description
Product Overview
SLS’ DNA Amplification Teaching Kit demonstrates how to set up the PCR reaction and operate the thermal cycler machine for the amplification of the desired gene fragment.
Principle
Polymerase Chain Reaction (PCR) is a widely used molecular biology technique used to rapidly generate millions to billions of copies of a specific DNA region. This process enables scientists to amplify even a very small DNA sample into a sufficient quantity for detailed analysis and study.
A basic PCR setup requires several essential components:
| Component | Description |
|---|---|
| DNA Template | Contains the target DNA region to be amplified. |
| DNA Polymerase | An enzyme responsible for synthesizing new DNA strands. |
| DNA Primers | Two primers complementary to the 3′ ends of the sense and anti-sense strands of the target DNA. |
| dNTPs | Building blocks used by DNA polymerase to synthesize new DNA strands. |
| Buffer Solution | Provides the optimal chemical environment for the activity and stability of DNA polymerase. |
The PCR reaction is commonly carried out in a volume of 10–200 μL using small reaction tubes placed inside a thermal cycler.
