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DNA Double Digestion kit

To perform double digestion of DNA and find out the molecular weight of each fragment of DNA on agarose gel along with a DNA molecular weight marker.

This DNA analysis kit demonstrates the procedures and principles of DNA Double digestion. Digestion of phage lambda DNA is followed by agarose gel electrophoresis.

Features

  • Fast digest restriction endonuclease enzymes for rapid and accurate digestion of the DNA Molecule
  • Use of two different enzymes to digest the sample DNA

Description

Product Overview

Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use two different restriction enzymes to digest genomic DNA from lambda bacteriophage. By visualizing the effects of two different enzymes on identical samples of double-stranded DNA, students learn that different restriction enzymes recognize and cut different DNA sequences.

Kit Contents

Materials Provided Storage on Receipt
Restriction Enzyme: Eco R1 -20°C
Restriction Enzyme: Hind III -20°C
Lambda DNA (uncut; as substrate) -20°C
10X Digestion Assay Buffer -20°C
Lambda DNA Eco/Hind Digest Marker -20°C
10X Gel Loading Buffer -20°C
Nuclease-Free Water -20°C

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at the above-defined temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Document Download
User Manual Download PDF
COA Download PDF
MSDS Download PDF

Ordering Information

Product Pack Size Catalog No. Price
Restriction Digestion kit 10 Preps SCMT006 Inquire
Restriction Digestion kit 20 Preps SCMT006A Inquire

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