• SLS’ RNA Extraction kit(mini) provide user friendly and easy protocol for purification of total RNA from small amount of starting material for various downstream processing.
• SLS’ RNA Extraction kit(mini) provides protocols for use with bacteria, Human blood and Yeast.
• This simple protocol which are ideal for simultaneous processing of multiple samples, yield pure RNA. This protocol requires no phenol/chloroform extraction or alcohol precipitation, and involves very little handling.
• The purified RNA is free of nucleases and other contaminants or inhibitors.

Principle

The RNA extraction kit protocol represents a well-established technology for RNA purification. This technology uses the selective binding properties of a silica-based membrane. A specialized high-salt buffer system allows up to 100 μg of RNA longer than 200 bases to bind to the silica membrane.

Biological samples are first lysed and homogenized in the presence of a highly denaturing chaotropic salt buffer, which immediately inactivates RNases to ensure purification of intact RNA. Ethanol is added to provide appropriate binding conditions, and the sample is then applied to an spin column, where the total RNA binds to the membrane and contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100 μl water.

Applications

• The SLS’ RNA Extraction kit (mini) is designed for the purification of total RNA from cultured cells. Other sample materials like blood, yeast and bacteria require an additional specific pre-lysis treatment, which is described in the Booklet
• Due to the integrated DNase digestion step, contamination of the isolated RNA with residual genomic DNA is mostly avoided. In addition, RNA is suited for other techniques like northern blotting, RNase protection and primer extension. Up to 24 samples can be processed simultaneously in approx. 1 hour. Thus, the purification procedure is less time consuming compared with alternative methods which require extraction with organic solutions, RNA precipitation or ultracentrifugation.
• The extraxted highly pure RNA may be used directly in RT-PCR and real-time RT-PCR, Differential display, cDNA synthesis, Northern, dot, and slot blot analyses, Primer extension, Poly A+ RNA selection, RNase/S1 nuclease protection, Microarrays.