Gene Expression – SLS Research

Gene Expression qPCR

Measure gene expression levels with high sensitivity and specificity. Quantify mRNA abundance for any target gene with real-time PCR technology.

Turnaround Time

2-3 weeks for complete analysis (Project dependent)

Sensitivity

High sensitivity and specificity

Flexibility

Any target gene or custom panel

Analysis

Fold change and statistical tests

Clearer Gene Expression Insights

Precise and reliable gene expression analysis with real-time qPCR technology.

High Sensitivity

Detect even low-level gene expression with precision

Rapid Results

Real-time monitoring during PCR amplification

Comprehensive Analysis

Fold changes, statistical tests, and interpretation

Our Process

From sample submission to comprehensive gene expression analysis

Sample Submission

Submit RNA samples with sample information

RNA Quality Check

Verify RNA integrity and concentration

cDNA Synthesis

Reverse transcribe RNA to complementary DNA

qPCR Analysis

Quantify gene expression using real-time PCR

Data Analysis

Calculate fold changes and statistical analysis

Report Delivery

Receive comprehensive expression data and analysis

Service Details

Explore our comprehensive DNA barcoding services.

Applications

Gene Validation:

Confirm microarray or RNA-seq results

Expression Profiling:

Measure mRNA levels across conditions

Disease Biomarkers:

Identify disease-associated gene expression

Drug Response:

Assess gene expression changes after treatment

Quality Control:

Verify sample quality and RNA integrity

Marker Selection

Sample Type:

Total RNA, mRNA, or tissue samples

RNA Quality:

RIN > 7 (preferably > 8)

Quantity:

Minimum 100 ng of total RNA

Storage:

80°C in RNase-free conditions

Deliverables

Ct Values:

Raw cycle threshold data for all samples

Fold Changes:

Relative expression levels compared to control

Statistical Analysis:

P-values and significance testing

Comprehensive Report:

Data visualization and interpretation

Frequently Asked Questions

How many genes can I analyze?

You can analyze any number of target genes. We recommend including 1-3 reference genes for normalization.

What reference genes do you use?

We use validated reference genes (GAPDH, ACTB, 18S rRNA) or can use your preferred reference genes.

Can you design custom primers?

Yes, we can design and validate custom primers for your target genes if needed.

What is the detection limit?

qPCR can detect expression levels as low as 1-10 copies per sample depending on primer efficiency.

Scientific Expertise You Can Rely On

Expert Analysis

Experienced team expertise in population genetics

Comprehensive Reports

Detailed analysis with statistical interpretation

Fast Turnaround

2-3 weeks from sample submission to results

“Highly accurate gene expression analysis with consistent qPCR results and clear data interpretation. The service was professional and reliable.”

— Research Scientist, Molecular Biology Laboratory

“High-quality RNA oligos with precise sequences and reliable stability. They worked efficiently in our downstream applications.”

— Laboratory Manager, Genomics Centre

“The oligos delivered excellent specificity with minimal background signal. The quality control documentation was clear and complete.”

— Project Lead, Biotechnology Institute

“Reliable RNA oligo synthesis with timely delivery and responsive technical support. The products fully met our research requirements.”

— Senior Researcher, Life Sciences Department

Ready to Measure Gene Expression?

Submit your RNA samples and receive comprehensive gene expression analysis with fold changes and statistical interpretation.