DNA Amplification kit

This kit demonstrates the amplification of desired fragment from the template DNA by Polymerase Chain Reaction(PCR).
Features:

  • Rapid and user friendly protocol
  • Helps the students to understand the concept and methodology of PCR
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Description

SLS’ DNA Amplification Teaching Kit demonstrates how to set up the PCR reaction and the thermal cycler machine for the amplification of desired gene fragment.

Principle:
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a speci?c Region of gene, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail.

A basic PCR set-up requires several components including:

  • A DNA template that contains the DNA target region to amplify
  • A DNA polymerase: an enzyme that polymerizes new DNA strands;
  • Two DNA primers that are complementary to the 3′ ends of each of the sense and anti-sense strands of the DNA target.
  • dNTPs- the building blocks from which the DNA polymerase synthesizes a new DNA strand
  • A Buffer Solution providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.

The reaction is commonly carried out in a volume of 10–200 μL in small reaction tubes in a thermal cycler.

Kit Contents

Materials Provide in kit Storage upon receipt
2X PCR Master Mix -20◦C
Taq DNA Polymerase -20◦C
Template DNA -20◦C
Nuclease Free Water -20◦C
Gel Loading Dye -20◦C
DNA Marker(100 bp Ladder) -20◦C

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.

insert / manual

Product Pack size Catlog No. Price
DNA Amplification kit (PCR Teaching kit 10 Preps SCMT011 inquire
DNA Amplification kit (PCR Teaching kit) 20 preps SCMT011A inquire