Product Overview

SLS’ DNA Amplification Teaching Kit demonstrates how to set up the PCR reaction and the thermal cycler machine for the amplification of desired gene fragment.

Features and Benefits

• Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a speci?c Region of gene, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail.

A basic PCR set-up requires several components including:

• A DNA template that contains the DNA target region to amplify
• A DNA polymerase: an enzyme that polymerizes new DNA strands;
• Two DNA primers that are complementary to the 3′ ends of each of the sense and anti-sense strands of the DNA target.
• dNTPs- the building blocks from which the DNA polymerase synthesizes a new DNA strand
• A Buffer Solution providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.
• A Buffer Solution providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.

The reaction is commonly carried out in a volume of 10–200 μL in small reaction tubes in a thermal cycler.