Safegene gDNA Purification kit ( Bacteria, Yeast, Fungi, Tissue Cultured cells)

Description

SLS’ Safegene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.

The simple Safegene procedure uses a modified precipitation method for purification of DNA. No mixing or dilution of solutions is necessary, and hands-on time is minimized. The procedure provides convenient stopping points that allow safe, temporary storage of partially purified samples.

Application

DNA purified using Safegene Kits is highly stable and suited for use in a wide range of applications, such as:

DNA archiving
PCR
SNP analysis
Southern blotting

Product Specifications

Elution volume	50–250 µl (varies)
Format	Scalable
Main sample type	Bacterial DNA / yeast DNA, Mouse Tail Kit, Blood, Cultured cells, Buccal brush, mouth wash, Tissue samples
Processing	Manual
Sample amount	Varies according to sample type
Technology	Modified precipitation method
Time per run or per prep	25–60 minutes (+ Cell Lysis + DNA Rehydration)

Kit Contents

Kit Contents	Storage Temperature (upon receipt)
Buffer CR	15-25°C
Buffer L	15-25°C
Buffer P	15-25°C
Buffer DE	15-25°C
Lytic Enzyme Solution	‘-20°C

Note that yields of genomic DNA will vary depending on bacterial strain, quality of the starting material, growing conditions, and the amount of material processed.

Document	Download
User Manual	Download PDF
COA	Download PDF
MSDS	Download PDF

Ordering Information

Product	Pack Size	Catalog No.	Price
Safegene gDNA Purification Kit	50 Preps	SCMR017	Inquire
Safegene gDNA Purification Kit	100 Preps	SCMR017A	Inquire
Safegene gDNA Purification Kit	200 Preps	SCMR017B	Inquire