Most PCR-amplified products obtained using a Taq-based DNA polymerase have a single base, deoxyriboadenosine (dA), added at the 3’ end. Among the methods for cloning PCR products with 3′-A overhangs, the TA cloning method utilizes a T-vector with a single deoxyribothymidine (dT) addition at the 3’ end. This cloning mechanism takes advantage of the complementarity between the single dT overhang of the vector and the single dA overhang at the end of the PCR product. The SLS’s TA-cloning Kit allows for fast, easy cloning of PCR products with this TA cloning method. The DNA Ligation Kit is used for the ligation reaction, enabling highly efficient ligations to be performed simply and quickly.